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Fluorescence Study Of Dyes
Part 1 of Paper
....isible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra.
For fluorescence spectra, there are two kinds, the excitation and the emission spectrum.
The ex.... Part 2 of Paper
....lmer's law and Förster's theory are obeyed.
2. Results
2.1. Electronic Absorption Spectra
We made up stock solutions for both salts and diluted them down to use in the UV/vis spectrometer.
Coumarin 1
C = 8 x10-5 mol dm -3
We obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573, using Beer-Lambert's law, we deduced the molar decadic absorption coefficients.
Sodium fluorescein
C = 2.425 x10-5 mol dm -3
Here max was 500.5 nm at an absorbance of 2.0922
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| Number of words: 815 - Approximate pages: 3 |
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