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Green Fluorescent Protein Purification
Part 1 of Paper
....cal tool with a wide range of applications in current research. The natural function of GFP is to facilitate the bioluminescence of jellyfish and other coelenterates. Aequorea GFP (from jellyfish) consists of 238 amino acids packed tightly together in a cylindrical structure called a beta-can (characterized by Yang, .... Part 2 of Paper
....physical characteristics to visualize various cellular activities.
One such application of GFP is to understand the role of a hormone, T3, in the translocation of thyroid hormone nuclear receptors (TRs).* In this project, GFP was fused to the human TR subtype b1 protein and the protein’s intracellular movement was visually tracked in live cells in the presence or absence of the T3 hormone. The prevalent hypothesis of this experiment was that T3 is essential in directing TR proteins to the nu.... |
| Number of words: 1280 - Approximate pages: 5 |
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