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Determination Of An Unknown Amino Acid From Titration
Part 1 of Paper
.... The initial pH of the solution was 1.96, and the pKa’s
found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were
found to be 2.10, 4.07, and 9.47. The molecular weight was calculated to be
176.3 while the accepted value was found to be 183.5. The identity of the
unknown amino acid was established.... Part 2 of Paper
....e glycine hydrochloride (see fig. 2). Glycine hydrochloride is an
example of an acidic salt form of the amino acid. Likewise, if NaOH were added,
the resulting amino acid would be sodium glycinate (see fig. 3), an example of a
basic salt form.
Fig. 2
Fig. 3
Due to the nature of amino acids, a titration curve can be employed to identify
an unknown amino acid. A titration curve is the plot of the pH versus the volume
of titrant used. In the case of amino acids, the titrant will be both an a.... |
| Number of words: 1634 - Approximate pages: 6 |
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